Table 1.
A: EMSA Probes. Positions of probes are given relative to TSP. Putative NRF-2 binding sites are underlined. | ||
---|---|---|
Gene Promoter |
Position | EMSA Sequence |
Gria1 | +198/+221 | F: 5′ TTTTAGAAGGAAGGGAGGAAGGAAAGAA 3′ |
R: 5′ TTTTTTCTTTCCTTCCTCCCTTCCTTCT 3′ | ||
Gria2 | −327/−298 | F: 5′ TTTTCGGCTTCCTAGGCATAGCAACCGGAAATCA 3′ |
R: 5′ TTTTTGATTTCCGGTTGCTATGCCTAGGAAGCCG 3′ | ||
Gria3 | +101/+117 | F: 5′ TTTTGGGTGGAAAGGAAGAGT 3′ |
R: 5′ TTTTACTCTTCCTTTCCACCC 3′ | ||
Gria4 | +403/+420 | F: 5′ TTTTTGGGGGAAAGGGAATGGG 3′ |
R: 5′ TTTTCCCATTCCCTTTCCCCCA 3′ | ||
COX6b | −47/−23 | F: 5′ TTTTTCCTCTTGCAGCTTCCGGCCAGTC 3′ |
R: 5′ TTTTGACTGGCCGGAAGCTGCAAGAGGA 3′ |
B: Mutant EMSA Probes. Positions of probes are given relative to TSP. Mutated NRF-2 binding sites are underlined. | ||
---|---|---|
Gene Promoter |
Position | Sequence |
Gria1 | +198/+221 | F: 5′ TTTTAGAATTTTGGGAGGTTTTAAAGAA 3′ |
R: 5′ TTTTTTCTTTAAAACCTCCCAAAATTCT 3′ | ||
Gria2 | −327/−298 | F: 5′ TTTTCGGCAAAATAGGCATAGCAACCTTTTATCA 3′ |
R: 5′ TTTTTGATAAAAGGTTGCTATGCCTATTTTGCCG 3′ |