Table 3.
A: Primers used for promoter cloning analysis. | ||
---|---|---|
Gene Promoter |
Position | Primer |
Gria2 | −947/+152 | F: 5′ CAGACGCGTCCCAAGCAGGCTCGGTGTAATGA 3′ |
R: 5′ CAGAGATCTGCTGTGGTCCCGGTGTCTGG 3′ | ||
COX6b | −291/+44 | F: 5′ TTGGTACCACTCTGCAGACAGCCTCAC |
R: 5′ TTAAGCTTCGGAGCAGCGTTACTTCAAT |
B: Primers used for promoter mutagenesis analysis. Mutated NRF-2 binding sites are underlined. | ||
---|---|---|
Gene Promoter |
Position | Primer |
Mut. NRF-2 Gria2 | −333/−288 | F: 5′ GCAGTTCGGCTGCTTAGGCATAGCAACCGTACATCAGTTTTGCAGC 3′ |
R: 5′ GCTGCAAAACTGATGTACGGTTGCTATGCCTAAGCAGCCGAACTGC 3′ | ||
Mut NRF-2 COX6b | −35/−32 | F: 5′ TCTCCTCTTGCAGCTAGAGGCCAGTCGGAATTCCG 3′ |
R: 5′ CGGAATTCCGACTGGCCTCTAGCTGCAAGAGGAGA 3′ |