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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Sep 22;1843(12):3018–3028. doi: 10.1016/j.bbamcr.2014.09.006

Table 3.

A: Primers used for promoter cloning analysis.
Gene
Promoter
Position Primer
Gria2 −947/+152 F: 5′ CAGACGCGTCCCAAGCAGGCTCGGTGTAATGA 3′
R: 5′ CAGAGATCTGCTGTGGTCCCGGTGTCTGG 3′
COX6b −291/+44 F: 5′ TTGGTACCACTCTGCAGACAGCCTCAC
R: 5′ TTAAGCTTCGGAGCAGCGTTACTTCAAT
B: Primers used for promoter mutagenesis analysis. Mutated NRF-2 binding sites are underlined.
Gene
Promoter
Position Primer
Mut. NRF-2 Gria2 −333/−288 F: 5′ GCAGTTCGGCTGCTTAGGCATAGCAACCGTACATCAGTTTTGCAGC 3′
R: 5′ GCTGCAAAACTGATGTACGGTTGCTATGCCTAAGCAGCCGAACTGC 3′
Mut NRF-2 COX6b −35/−32 F: 5′ TCTCCTCTTGCAGCTAGAGGCCAGTCGGAATTCCG 3′
R: 5′ CGGAATTCCGACTGGCCTCTAGCTGCAAGAGGAGA 3′