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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Apoptosis. 2014 Nov;19(11):1616–1626. doi: 10.1007/s10495-014-1029-5

Fig. 2. DAPT enhances VCR-induced apoptosis in T-ALL.

Fig. 2

GSI-resistant NOTCH1 mutant TALL lines (a, Jurkat; b, CEM; c, P12), GSI-sensitive NOTCH1 mutant T-ALL lines (d, KOPT; e, HSB-2), and a GSI-resistant wild-type NOTCH1 T-ALL line (f, Loucy) were treated with varying concentrations of VCR (1–3 nM) and/or DAPT (10 μM) as indicated for 48 h. Annexin V (AV) and Propidium Iodide (PI) binding was measured by flow cytometry. The percentage of viable cells (AV-PI-), early apoptotic cells (AV+PI-), and late apoptotic cells (AV+PI+) is graphed. All results are presented as mean ± SD of triplicate assays. The statistical significance of differences was determined by ANOVA test; *, p < 0.05; **, p < 0.01; ***, p< 0.001