Figure 1.

Analysis of the AAO3-GFP fusion protein by native PAGE. A, Zymograms of aldehyde oxidases (AOs) from Arabidopsis wild-type (WT), aao3 mutant (aao3), and AAO3 promoter:AAO3-GFP-introduced aao3 (a1-1 and a4-8) plants. Enzyme extracts were subjected to native PAGE, and activity bands were developed using abscisic aldehyde as the substrate. Each lane was loaded with 50 μg of protein. B, Immunoblotting with the same extracts as in A. After native PAGE, immunoblotting was performed using anti-AAO3 (left) and anti-GFP (right) antibodies. Black and white arrows indicate the activity bands of aldehyde oxidase isoforms in the WT and AAO3-GFP transgenic plants, respectively. Dotted arrows indicate the position of AOγ.