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Preparation of AAO3-specific antibodies. Immunoblot analysis with various mouse antisera against AAO3 protein. Recombinant AAO1, AAO2, and AAO3 proteins were subjected to native PAGE followed by immunoblot analysis using the I1 to I10 immune serum obtained from 10 individually AAO3-injected mice. Lanes 1, 2, and 3 contain recombinant purified AAO1, AAO2, and AAO3 protein, respectively. Each lane contains 100 ng of purified protein.
