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. 2004 Apr;134(4):1697–1707. doi: 10.1104/pp.103.036970

Figure 8.

Figure 8.

Detection of AAO3 mRNA in guard cells by in situ hybridization. Sections of epidermis from water-stressed or nonstressed leaves were hybridized with AAO3 sense and antisense RNA probes. AAO3 mRNA-positive signals are dark blue. The AAO3 RNA sense probe was used as a negative control. A, Hybridization of the AAO3 antisense probe to a 0-h stressed leaf. B, Hybridization of the AAO3 antisense probe to a well-watered leaf. C, Hybridization of the AAO3 antisense probe with a 3-h stressed leaf. D, Hybridization of the AAO3 sense probe with a 3-h stressed leaf. Bars = 10 μm.