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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Stem Cells. 2014 Nov;32(11):3023–3030. doi: 10.1002/stem.1799

Figure 2. Acute LCMV infection induces innate immune response and promotes myelopoiesis.

Figure 2

Wild type animals were infected by IP injection with 1 × 105 pfu LCMV Armstrong. (A) The levels of IFNα and IFNγ in serum were measured by ELISA at 2-day intervals over the first 8 days following infection. n=3 per time point. (B) Hematopoietic stem and progenitor cells (SP, Lin−) increased in marrow of infected WT animals, peaking at 6 days following LCMV infection in WT animals. n=3 per time point. (C) 1 × 104 WBM cells from naïve or LCMV infected animals were cultured in methylcellulose media for 12 days. Bars show numbers of total colonies. n=6. (D) Complete blood counts for peripheral blood of naïve or LCMV infected WT animals were compared on day 6 post-infection. The monocyte count was significantly increased. n=3 (E) After LCMV infection, WBC count peaked at 8 days post-infection, just after the increase seen in progenitors and CFUs. Each graph is representative of 2–3 experiments. *p<0.05, *** p<0.001, n.s. not significant.