Fig. 2.

Solution and spectroscopic properties of FAPol_AF647. a Elution profiles at 220 nm (solid line) and 650 nm (dashed line) of crude FAPol_AF647. The concentration injected was 5 g L⁻. Elution buffer: 0.1 M NaCl, 20 mM Tris/HCl, pH 8. au arbitrary units, V_el elution volume, V₀ excluded volume, V_T total volume. b Elution profiles at 220 nm (solid line) and 650 nm (dashed line) of purified FAPol_AF647. Same conditions. The profiles were normalized to the same maximum. c Absorption spectrum of 0.1 g L⁻ FAPol_AF647 in the same buffer. d Emission (solid line) and excitation (dashed line) spectra of a 0.001 g L⁻ solution of purified FAPol_AF647; same buffer