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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Membr Biol. 2014 May 25;247(0):957–964. doi: 10.1007/s00232-014-9669-5

Table 1. Different conditions tested for amphipol refolding of BR. Letters in top row represent labels of resulting SEC profiles as shown in Figure 1.

b c1 d2 e f g h i j k l m3
bacterioOpsin + + + + + + + + + + + +
Amphipol - + + + + + + + + + + +
Retinal present - + + - - + + + + + + +
Bound to Ni-NTA - - - - + - + - + - + -
KCl-precipitate + + + + +
Dilute + +
Bio-Beads + +
1

after Ni-NTA purification with amphipol-free buffer

2

same as c) but after 12 h incubation with free amphipols

3

reinjection after first SEC separation (using method in g) and collection of BR containing fraction