Table 1. Re-screening data on potential agonists or antagonists identified in the initial chemical screen.
Regulator Name | Concentration #1 (μM) | Hepcidin-Luciferase Activity at Conc. #1 Mean±SE (AU) N=2 | Concentration #2 (μM) | Hepcidin-Luciferase Activity at Conc. #2 Mean±SE (AU) N=2 | Concentration #3 (μM) | Hepcidin-Luciferase Activity at Conc. #3 Mean±SE (AU) N=2 |
---|---|---|---|---|---|---|
POTENTIAL AGONISTS | ||||||
Vorinostat | 33 | 56.08±35.27 | 6.6 | 48.14±6.70 | 1.3 | 14.60±4.68 |
9-aminoacridine | 33 | 49.76±12.27 | 6.6 | 1.14±0.40 | 1.3 | 0.68±0.07 |
Acrisorcin | 33 | 29.85±1.50 | 6.6 | 0.99±0.06 | 1.3 | 0.77±0.00 |
Ethacridine lactate | 33 | 15.18±1.13 | 6.6 | 1.35±0.24 | 1.3 | 1.12±0.18 |
Doxorubicin | 12 | 11.39±0.49 | 2.4 | 0.68±0.06 | 0.5 | 0.77±0.02 |
Daunorubicin | 12 | 11.33±0.07 | 2.4 | 0.99±0.05 | 0.5 | 0.67±0.05 |
Leflunomide | 33 | 5.50±0.29 | 6.6 | 3.10±0.23 | 1.3 | 1.12±0.18 |
#5322770 | 66 | 5.03±0.21 | 12.2 | 1.42±0.04 | 2.6 | 0.84±0.06 |
Ipriflavone | 33 | 4.57±0.30 | 6.6 | 3.41±0.08 | 1.3 | 2.56±0.07 |
Lansoprazole | 33 | 4.09±0.44 | 6.6 | 0.98±0.01 | 1.3 | 0.58±0.01 |
Pterostilbene | 33 | 4.06±0.59 | 16.5 | 3.91±0.28 | 8.25 | 2.21±0.19 |
Amlexanox | 33 | 3.84±0.95 | 6.6 | 2.65±0.06 | 1.3 | 0.67±0.02 |
Nabumetone | 33 | 3.74±0.19 | 16.5 | 3.16±0.30 | 8.25 | 3.61±0.95 |
Topotecan | 33 | 3.63±1.39 | 6.6 | 0.57±0.05 | 1.3 | 0.73±0.04 |
Camptothecin | 33 | 3.62±0.62 | 6.6 | 1.55±0.34 | 1.3 | 0.60±0.05 |
Chrysin | 26 | 3.53±0.60 | 5.2 | 1.09±0.11 | 1.0 | 0.76±0.01 |
GTP-14564 | 11 | 3.07±0.60 | 2.2 | 1.35±0.07 | 0.4 | 1.17±0.15 |
10058-F4 | 33 | 2.92±0.23 | 6.6 | 2.17±0.01 | 1.3 | 1.25±0.13 |
SB 204741 | 33 | 2.87±0.22 | 6.6 | 1.96±0.18 | 1.3 | 1.09±0.04 |
Phenazopyridine | 26 | 2.85±0.04 | 5.2 | 1.51±0.08 | 1.0 | 0.95±0.12 |
AS-252424 | 33 | 2.84±0.02 | 6.6 | 1.40±0.03 | 1.3 | 0.73±0.02 |
AG 1296 | 11 | 2.41±0.14 | 2.2 | 1.03±0.11 | 0.4 | 0.90±0.07 |
SP 600125 | 33 | 1.86±0.00 | 6.6 | 1.11±0.02 | 1.3 | 0.93±0.09 |
Fenbendazole | 22 | 1.75±0.08 | 4.4 | 1.03±0.20 | 0.9 | 0.96±0.12 |
#5105276 | 66 | 1.73±0.04 | 12.2 | 0.75±0.13 | 2.6 | 0.74±0.02 |
GW9662 | 33 | 1.66±0.08 | 6.6 | 1.12±0.05 | 1.3 | 1.09±0.01 |
#5102420 | 66 | 1.66±0.06 | 12.2 | 0.80±0.11 | 2.6 | 0.83±0.16 |
Imatinib | 33 | 1.24±0.13 | 16.5 | 1.18±0.06 | 8.25 | 1.31±0.06 |
Nocodazole | 22 | 1.13±0.02 | 4.4 | 0.84±0.11 | 0.9 | 0.84±0.01 |
#5193825 | 66 | 1.13±0.14 | 12.2 | 0.67±0.01 | 2.6 | 0.69±0.06 |
POTENTIAL ANTAGONISTS | ||||||
#5215082 | 66 | 1.09±0.08 | 12.2 | 0.87±0.04 | 2.6 | 0.79±0.02 |
#5146972 | 66 | 1.08±0.00 | 12.2 | 0.72±0.05 | 2.6 | 0.66±0.03 |
SU-6668 | 33 | 0.31±0.02 | 6.6 | 0.67±0.09 | 1.3 | 0.88±0.01 |