. Author manuscript; available in PMC: 2016 Jan 1.

Published in final edited form as: J Biomed Mater Res A. 2014 Apr 23;103(1):409–419. doi: 10.1002/jbm.a.35188

Table 1.

Cytotoxic activity (IC₅₀) of TAT-Gel

Cell Line		IC50^a, nM
Cell Line		rGel	TAT-Gel	Fold Difference
Cancer Cells	LS174T	5130 ± 802	29.0 ± 15.0^***	177
	CT26	4190 ± 1120	72.2 ± 26.6^**	58
	U87 MG	3100 ± 409	46 ± 13.7^***	67
	9L	4260 ± 856	57.8 ± 26.3^**	74
	HeLa	4980 ± 920	66.4 ± 31.4^***	75
	PC-3	3330 ± 761	63.4 ± 25.1^**	53

Non Cancer Cells	MDCK	3580 ± 846	53 ± 16.3^**	68
Non Cancer Cells	293 HEK	4300 ± 1020	46.7 ± 14.0^**	92

IC₅₀ values were calculated by nonlinear regression using Prism software (GraphPad). All experiments were performed with three different batches (N=3).

^**

P < 0.001

^***

P < 0.0001 by Student's t-test. (rGel: recombinant gelonin, TAT-Gel: recombinant TAT-gelonin fusion protein)