Mean dry shoot mass and coefficient of variation (CV) in P, F1 and F2 of the combination Col-0/C24. A, Mean dry shoot mass at 15 DAS (left axis) and 28 DAS (right axis). Means of at least 16 plants ± sd are shown. B, Coefficients of variation at 15 and 28 DAS. The analysis shows the defining characteristics of heterosis; superior performance of the F1, and reduction of the effect in the F2. For comparisons between F1 and parents, plants grown from manually pollinated seeds were used, comparisons of the F2 and the parents were done with plants from self-pollinated seeds.