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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Neurobiol. 2014 Apr 15;51(1):43–56. doi: 10.1007/s12035-014-8691-z

Fig 8. Therapeutic rAAV9-IgVL5D3 injection decreases microglia/monocyte activation.

Fig 8

Five months after rAAV9 or PBS injection, activated microglia and/or monocytes in the right hippocampus and neocortex were visualized by immunohistochemistry using anti-Iba1 antibody (a) and quantified by morphometric analysis. The percentages of Iba1-immunoreactive areas are shown as bar graphs (b). Homogenates of the left hippocampi and neocortices were subjected to SDS-PAGE followed by immunoblotting using anti-CD45 antibody (c). The bar graph represents the optical density ratio to that of the GAPDH band on the same lane (d). Pro-inflammatory cytokine levels in the left neocortex homogenates were determined by ELISA (e). The values shown are the mean ± SEM. Scale bars, 1 mm.