Figure 3.

Adult-specific knockdown of fezf2 activity leads to NSC overproliferation. A, A schematic diagram describing the injection of fezf2 vivo MO into adult forebrain ventricles. B, C, Adult DTel from vivo MO-injected brains labeled with BLBP (B,C), PCNA (B′,C′), and DAPI (merged images B″,C″) shows increased expression of the proliferation marker PCNA (C′) compared with control (B′). D, Quantification shows an increase of BLBP⁺PCNA⁺ proliferative NSCs at the expense of BLBP⁺PCNA⁺ quiescent NSCs. N = 3 for both Ctrl MO group and fezf2 vivo MO group. Values represent mean ± SEM. **p < 0.01 (Student's t test).