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. 2014 Jan 25;6(4):485–498. doi: 10.1159/000357618

Fig. 2.

Fig. 2

Expression profile of tirS. a Expression of MSSA476 tirS in BHI was evaluated at OD600 0.3, 0.6, 0.9 and 1.2 by RT-PCR. b Expression of MSSA476 tirS in DMEM in the absence (×) or presence (◼) of HaCaT cells using S. aureus MSSA476 harboring tirS-GFP reporter construct. c Presence of TirS in both the lower (bacteria only) and upper compartment (HEK293 only) of a Transwell system as detected by immunoblot. d Presence of the cytoplasmic protein gyrase A in bacteria but not the supernatant of the lower compartment as assessed by immunoblot and gyrA-specific antibody.