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The ulp1-I615N mutant accumulates Smt3 protein conjugates. The images show Ponceau red staining of proteins after transfer onto a nitrocellulose membrane (left) and immunodetection of Smt3 conjugates with an anti-Smt3 antibody (right). Extracts (45 μg) were obtained from cells growing exponentially at 28°C. WT, wild type.
