TABLE 2.
Genetic interactions of the ulp1-I615N mutation with mutated genes (x) of the repair and recombination pathways
| Diploid heterozygous for ulp1-I615N and another mutation (no. of tetrads analyzed) | No. of monosporic clones
|
Nature of genetic interaction with ulp1 | |||
|---|---|---|---|---|---|
| Wild type | ulp1 single mutant | x single mutant | ulp1 x double mutant | ||
| Control: ulp1 × wild type (32) | 64 | 58 | |||
| Mutants specifically deficient in homologous recombination | |||||
| ulp1 × srs2Δ (48) | 44 | 45 | 39 | 1 | Colethal |
| ulp1 × rad51Δ (76) | 66 | 78 | 76 | 2 | Colethal |
| ulp1 × rad52Δ (48) | 46 | 41 | 38 | 3 | Colethal |
| ulp1 × rad54Δ (58) | 57 | 44 | 45 | 1 | Colethal |
| ulp1 × rad55Δ (21) | 10 | 23 | 21 | 1 | Colethal |
| Mutants deficient in both homologous recombination and NHEJ | |||||
| ulp1 × rad50Δ (59) | 59 | 43 | 48 | 0 | Colethal |
| ulp1 × mre11Δ (12) | 10 | 13 | 12 | 0 | Colethal |
| Mutant specifically deficient in NHEJ: ulp1 × yku70Δ (18) | 12 | 20 | 18 | 12 | Viable |
| Mutant mainly deficient in SSA (and homologous recombination): ulp1 × rad59Δ (32) | 28 | 25 | 23 | 27 | Viable |
| Mutants deficient in control of translesional bypass by ubiquitination | |||||
| ulp1 × rad18Δ (64) | 57 | 51 | 48 | 51 | Viable |
| ulp1 × rad6Δ (37) | 39 | 29 | 33 | 28 | Viable |
| Mutant deficient in excision repair: ulp1 × rad14Δ (22) | 21 | 13 | 18 | 21 | Viable |
| Mutant deficient in checkpoint entry: ulp1 × rad53/sad1-1 (31) | 26 | 24 | 32 | 21 | Viable |
| Mutant deficient in replication and repair: ulp1 × rad27Δ (17) | 17 | 16 | 14 | 0 | Colethal |