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Expression of PES1 and UBF1 in 32D-derived cells. 32D, 32D IGF-IR, 32D IGF-IR/IRS-1, and 32D IRS-1 cells were infected with retroviruses expressing either PES1 or UBF1 as described in Materials and Methods. Mixed populations were collected, lysates were made, and Western blots were developed with an antibody to the FLAG epitope. Parental 32D cells were used as negative controls for the FLAG antibody. The positions of ectopic PES1 (A) and UBF1 (B) are indicated by arrows.
