FIG. 2.

Growth curves for 32D-derived cell lines. The growth of the cell lines in either IGF-I (50 ng/ml) or IL-3 was determined. Open bars indicate parental cells, either 32D IGF-IR cells (upper panels) or 32D IGF-IR/IRS-1 cells (lower panels). The number of cells was counted at 24, 48, and 96 h after shifting to IGF-I or replating in IL-3. The numbers on the ordinate represent the percent increase over the number of plated cells (e.g., 300 indicates a threefold increase). Note the different ordinate scales for 32D IGF-IR and 32D IGF-IR/IRS-1 cells. We omitted an analysis of parental 32D cells, which grow very well in IL-3 and die quickly after a shift to IGF-I. Error bars indicate standard deviations.