FIG. 5.

Differentiation of 32D-derived cells into granulocytes. The cells examined were 32D IGF-IR cells, 32D IGF-IR cells expressing PES1, 32D IGF-IR cells expressing UBF1, and 32D IGF-IR/IRS-1 cells. The cells were stained with Giemsa stain 6 days after a shift from IL-3 to IGF-I. All images were taken at the same magnification, ×40. The arrows in panels A, B, and C indicate differentiating cells (bilobed, irregular nuclei and some well-differentiated granulocytes). The 32D IGF-IR/IRS-1 cells in panel D have round nuclei, and one of them (vertical arrow) is actually in mitosis. Note the difference in size between parental 32D IGF-IR cells (B) and the same cells expressing PES1 (C), UBF1 (A), or IRS-1 (D).