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. 2004 Jun;24(12):5421–5433. doi: 10.1128/MCB.24.12.5421-5433.2004

FIG. 8.

FIG. 8.

Expression of endogenous PES1 in 32D-derived cells. (A) 32D IGF-IR and 32D IGF-IR/IRS-1 cells were shifted from IL-3 to IGF-I, and the levels of endogenous PES1 expression in whole-cell lysates were measured. The time after the shift is indicated above the lanes in hours. PES1 expression persisted in transformed 32D IGF-IR/IRS-1 cells but not in differentiating 32D IGF-IR cells. (B) Expression of exogenous UBF1 and PES1 in differentiated 32D-derived cells. The cell lines examined were 32D IGF-IR cells overexpressing either PES1 or UBF1 3 days after a shift to IGF-1. Lanes IL-3 contained the same cells at zero time. Western blotting was done with antibodies to PES1 or UBF1. An anti-Grb2 antibody was used to monitor protein amounts.