FIG. 3.

Elevation of ACTR promotes cell cycle progression in antiestrogen-treated quiescent cells. (A) Schematics of the time course for treatment of T-47D cells for cell cycle analysis by FACS (see Materials and Methods for details). (B) Two-dimensional FACS distribution of T-47D cells treated as above, grown in regular medium (Regular), or hormone deprived for 60 h (HD), or treated with 10⁻⁷ M E2 (E2 48 h), and then labeled with BrdU for 10 h prior to harvesting and staining with propidium iodide. The horizontal axis in the dot plots reflects DNA content, and the vertical axis shows DNA synthesis. The populations of cells in G₁ and S phases are indicated. Cells mock infected showed no difference in cell cycle distribution from cells infected with adeno-GFP (data not shown).