Figure 3. Stages III and IV: The anti-SP-10 antibody staining distinguishes round spermatids of stages III and IV.
The anti-SP-10 antibody shows the round shape of the acrosome of round spermatids at stage III (St3 in A), and its triangular shape at stage IV (St4 in B). In contrast, PAS staining can only be seen in round spermatids at stage IV (St4 in D). In, intermediate spermatogonium; P, pachytene spermatocyte; Ser, Sertoli cell; St3 and St4, Step-3 and Step-4 round spermatids; St15, Step-15 elongated spermatids. Scale bars, 50 µm.