Table 2.
Differentially expressed proteins between the healthy and mastitis-infected cows’ mammary gland tissues
| Accession | Protein names | Official gene name | Peptides (95%) | Fold-change (114:117) | P value 114:117 |
|---|---|---|---|---|---|
| Up-regulated proteins in the mastitis group | |||||
| tr|F1MGW0 | Collagen alpha-1(I) chain | COL1A1 | 177 | 3.70 | 0.0001 |
| tr|E1BB91 | Collagen alpha-3(VI) chain | COL6A3 | 100 | 2.99 | 0.0000 |
| sp|P02465 | Collagen alpha-2(I) chain | COL1A2 | 100 | 3.56 | 0.0002 |
| sp|P02769 | Serum albumin OS | ALB | 89 | 2.27 | 0.0000 |
| tr|F1N169 | Filamin-A | FLNA | 51 | 2.61 | 0.0000 |
| tr|F1MQ37 | myosin-9 | MYH9 | 49 | 2.23 | 0.0010 |
| tr|F1N4K8 | ENSBTAG00000002278 | FBN1 | 31 | 3.19 | 0.0000 |
| tr|E1BI98 | Collagen alpha-1(VI) chain | COL6A1 | 30 | 2.58 | 0.0026 |
| tr|D4QBF3 | Hemoglobin beta | HBB | 25 | 3.50 | 0.0000 |
| tr|F1MKG2 | collagen alpha-2(VI) chain precursor | COL6A2 | 22 | 2.31 | 0.0032 |
| sp|P02676 | Fibrinogen beta chain | FGB | 22 | 11.48 | 0.0000 |
| tr|A5PJE3 | Fibrinogen alpha chain | FGA | 22 | 8.87 | 0.0000 |
| sp|O62654 | Desmin | DES | 20 | 7.24 | 0.0000 |
| sp|Q7SIH1 | Alpha-2-macroglobulin | A2M | 18 | 3.50 | 0.0000 |
| tr|F1MER7 | Basement membrane-specific heparan sulfate | HSPG2 | 17 | 1.77 | 0.0180 |
| sp|Q9TTE2 | Decorin | DCN | 17 | 2.29 | 0.0109 |
| sp|Q27991 | Myosin-10 | MYH10 | 16 | 3.10 | 0.0386 |
| sp|Q9TTE1 | Serpin A3-1 | SERPINA3-1 | 15 | 3.34 | 0.0216 |
| tr|B0FZM4 | Myosin light chain 6 | MYL6 | 15 | 1.67 | 0.0106 |
| tr|B8Y9T0 | Cumulus cell-specific fibronectin 1 transcript variant | FN1 | 14 | 6.92 | 0.0000 |
| tr|Q3SZZ9 | Fibrinogen gamma-B chain | FGG | 14 | 16.75 | 0.0000 |
| tr|Q5EA67 | Inter-alpha (Globulin) inhibitor H4 | ITIH4 | 10 | 4.49 | 0.0000 |
| tr|F1MIQ8 | Biglycan | BGN | 9 | 2.54 | 0.0133 |
| sp|P79134 | Annexin A6 | ANXA6 | 8 | 2.00 | 0.0233 |
| sp|P01967 | Hemoglobin subunit alpha-1 | HBA1 | 8 | 4.41 | 0.0020 |
| sp|Q2TBU0 | Haptoglobin | HP | 7 | 5.45 | 0.0020 |
| sp|P79105 | Protein S100-A12 | S100A12 | 6 | 11.17 | 0.0002 |
| sp|Q2KJH6 | Serpin H1 | SERPINH1 | 6 | 3.05 | 0.0040 |
| tr|F1MHS5 | Protein S100-A9 | S100A9 | 6 | 5.01 | 0.0097 |
| tr|E1B6Z6 | Neutrophil gelatinase-associated lipocalin | LCN2 | 5 | 3.56 | 0.0049 |
| tr|F1N076 | ENSBTAG00000012164 | CP | 4 | 5.81 | 0.0129 |
| sp|P51176 | Protein-glutamine gamma-glutamyltransferase 2 | TGM2 | 4 | 8.32 | 0.0069 |
| sp|P28782 | Protein S100-A8 | S100A8 | 4 | 5.01 | 0.0499 |
| tr|A3KN02 | HIST1H1C protein | HIST1H1C | 3 | 1.69 | 0.0011 |
| tr|F1N3A1| | Thrombospondin-1 | THBS1 | 3 | 3.08 | 0.0434 |
| tr|F1MTV8 | ENSBTAG00000047345 | ENSBTAG00000047345 | 2 | 21.68 | 0.0455 |
| Down-regulated proteins in the mastitis group | |||||
| sp|P02662 | Alpha-S1-casein | CSN1S1 | 65 | 0.10 | 0.0011 |
| tr|F1N2D9 | Uncharacterized protein | F1N2D9 | 45 | 0.48 | 0.0000 |
| tr|B5B0D4 | Heat shock protein 90 kDa beta member 1 | HSP90B1 | 32 | 0.12 | 0.0000 |
| tr|Q9N273 | Kappa-casein (Fragment) | CSN3 | 27 | 0.17 | 0.0018 |
| sp|P02663 | Alpha-S2-casein | CSN1S2 | 25 | 0.19 | 0.0001 |
| tr|F1MU12 | Keratin, type II cytoskeletal 8 | KRT8 | 24 | 0.27 | 0.0000 |
| sp|P08728 | Keratin, type I cytoskeletal 19 | KRT19 | 18 | 0.27 | 0.0024 |
| tr|F1MLK0 | Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial | IDH1 | 14 | 0.52 | 0.0036 |
| sp|P17248 | Tryptophanyl-tRNA synthetase, cytoplasmic | WARS | 10 | 0.41 | 0.0019 |
| sp|Q5E946 | Protein DJ-1 | PARK7 | 7 | 0.21 | 0.0096 |
| sp|P00727-2 | Isoform 2 of Cytosol aminopeptidase | LAP3 | 7 | 0.58 | 0.0465 |
| sp|P20000 | Aldehyde dehydrogenase, mitochondrial | ALDH2 | 7 | 0.43 | 0.0337 |
| sp|P81265 | Polymeric immunoglobulin receptor | PIGR | 6 | 0.20 | 0.0081 |
| sp|P42899 | 60S acidic ribosomal protein P2 | RPLP2 | 5 | 0.35 | 0.0069 |
| tr|F1N3Q7 | Apolipoprotein A-IV | APOA4 | 5 | 0.24 | 0.0129 |
| sp|P51977 | Retinal dehydrogenase 1 | ALDH1A1 | 5 | 0.34 | 0.0146 |
| tr|F1MDK4 | Ethylmalonyl-CoA decarboxylase | ECHDC1 | 5 | 0.19 | 0.0108 |
| tr|F1MZQ4 | Butyrophilin, subfamily 1, member A1 | BTN1A1 | 3 | 0.17 | 0.0052 |
| tr|C4T8B4 | C-reactive protein | CRP | 3 | 0.25 | 0.0027 |