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. 2014 Jun 17;16(5):441–450. doi: 10.1016/j.neo.2014.04.007

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© 2014 Neoplasia Press, Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

PMC Copyright notice

RNAi knockdown of CSNK1E (A and B) and CTNNB1 (C and D) selectively killed TP53^−/− cells. Cell viability was measured by MTT assay. Relative mRNA expression level was measured using the comparative CT (ΔΔCT) method; beta-actin mRNA was used as an internal control. Relative viability of HCT-116 and RKO cells (TP53^WT and TP53^-/-) transfected with (A) CSNK1E-targeting shRNAs (#1 and #2) or (C) CTNNB1-targeting shRNAs (#1 and #2), and control shRNA. Relative mRNA level of (B) CSNK1E following transfection of CSNK1E-targeting shRNAs (#1 and #2) or (D) CTNNB1 following transfection of CTNNB1-targeting shRNAs (#1 and #2), and control shRNA. Relative viability of cells (TP53^WT and TP53^-/-) following treatment with IC261 in (E) HCT-116 and (F) RKO. Relative viability of cells (TP53^WT and TP53^-/-) following treatment with D4476 in (G) HCT-116 and (H) RKO.