Table 4.

Overall survival of 171 CRC patients in relation to clinical factors and IHC of protein pairs.

A. Univariate Cox regression
Variable	Subset	Hazard ratio (95% CI)	P value
Age	age > 65/age < = 65	1.19 (0.86-1.66)	0.289
Gender	male/female	1.17 (0.84-1.63)	0.351
Grade	poor/well and moderate	1.27 (0.67-2.42)	0.466
Stage	III-IV/I-II	1.42 (1.03-1.97)	0.033
(MSH2(C), RB1(N))	(↓,↓)*/otherwise	4.34 (1.58-11.95)	0.015
(CSNK1E(C), P53(N))	(↓,↑)/otherwise	1.62 (1.15-2.26)	0.006
(CTNNB1(N), P53(N))	(↑,↑)/otherwise	1.50 (1.07-2.09)	0.018
(BRCA1(C), WNT5A(C))	(↓,↑)/otherwise	1.64 (1.02-2.63)	0.047
B. Multivariate Cox regression†
Variable	Subset	Hazard ratio (95% CI)	P value
Stage	III-IV/I-II	1.58 (1.11-2.24)	0.010
(MSH2(C), RB1(N))	(↓,↓)*/otherwise	7.53 (2.25-25.24)	0.001
(CSNK1E(C), P53(N))	(↓,↑)/otherwise	1.65 (1.14-2.38)	0.007
(CTNNB1(N), P53(N))	(↑,↑)/otherwise	1.50 (1.05-2.14)	0.027
(BRCA1(C), WNT5A(C))	(↓,↑)/otherwise	1.93 (1.17-3.19)	0.010

^*The symbols “↑”and “↓”denote abnormal IHC (overexpression and underexpression, respectively) of the corresponding protein.

^†Variables were selected by using stepwise selection method.