Figure 7.

Sustained expression of PEDF halts in vivo dissemination of melanoma cells induced by MITF silencing. (A) Fluorescent micrographs of the posterior tail showing distal dissemination of tumor cells (red) and their close association with the blood vessels of the embryo (green) 3 days after melanoma cell implantation. Size bar indicates 100 micrometers. White arrowheads indicate disseminated tumor cells. (B) The box-plots represent the number of distally disseminated tumor cells per fish (n = 21-25 fish/condition), 3 days after melanoma cell implantation. Statistical significance was determined by one-way ANOVA using Tukey-Kramer post-test (***P < 0.001).