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. 2014 Jun 24;16(6):511–528. doi: 10.1016/j.neo.2014.05.009

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© 2014 Neoplasia Press, Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

PMC Copyright notice

DAC reduces hTERT expression through decreased binding of c-myc to the hTERT promoter in CML cells.

K-562 cells were cultured in presence or absence of 2 μM DAC. (A) ChIP analysis of H3K4Me3 and c-myc recruitment to hTERT promoter. (B and C) Analysis of c-myc expression at protein and mRNA levels. (D) c-myc and hTERT expression at mRNA and protein levels in K-562 cells transfected with or without control siRNA or c-myc siRNAs. (E and F) Analysis of CDKN2D (p19) expression at protein and mRNA levels. (G) CDKN2D and c-myc expression were evaluated at mRNA and protein levels in K-562 cells transfected with or without control siRNA or CDKN2D siRNAs. Data are the mean (±SD) of three independent experiments. Blots are representative of three independent experiments. * P < 0.05, ** P < 0.01 versus control (one-way ANOVA).

DAC reduces hTERT expression through decreased binding of c-myc to the hTERT promoter in CML cells.

K-562 cells were cultured in presence or absence of 2 μM DAC. (A) ChIP analysis of H3K4Me3 and c-myc recruitment to hTERT promoter. (B and C) Analysis of c-myc expression at protein and mRNA levels. (D) c-myc and hTERT expression at mRNA and protein levels in K-562 cells transfected with or without control siRNA or c-myc siRNAs. (E and F) Analysis of CDKN2D (p19) expression at protein and mRNA levels. (G) CDKN2D and c-myc expression were evaluated at mRNA and protein levels in K-562 cells transfected with or without control siRNA or CDKN2D siRNAs. Data are the mean (±SD) of three independent experiments. Blots are representative of three independent experiments. * P < 0.05, ** P < 0.01 versus control (one-way ANOVA).