Table 1.
Acylation of ara-C with VL by P. fluorescens in IL-containing systems a
| Entry | Solvents b,c | V 0 (mmol/L · h) | Y(%) | 5′-regioselectivity(%) |
|---|---|---|---|---|
| 1 | THF | 2.42 | 4.6 | >99.0 |
| 2 | [EMI][BF4]/THF | 0.05 | 2.4 | >99.0 |
| 3 | [BMI][BF4]/THF | 0.03 | 3.2 | >99.0 |
| 4 | [HMI][BF4]/THF | 0.23 | 4.8 | >99.0 |
| 5 | [OMI][BF4]/THF | 0.25 | 7.5 | >99.0 |
| 6 | [BMI][PF6]/THF | 2.34 | 81.1 | >99.0 |
| 7 | [BMI][Br]/THF | 0 | 0 | 0 |
| 8 | [BMI][Cl]/THF | 0 | 0 | 0 |
| 9 | [BMI][TF2N]/THF | 3.15 | 70.4 | >99.0 |
aThe reaction conditions: 50 mg/mL biomass, 20 mmol/L ara-C, 500 mmol/L VL, 40 μL water, 1 mL solvents, 40°C, 200 rpm and 144 h. bIL content, 10% v/v; cPyridine (10%, v/v) was added to the reaction media as an assistant solubilizer for dissolving the substrate.