Table 1.
PCR and genotyping details.
| Gene | Polymorphism, region | Primer details | PCR product (bp) | Annealing temperature | Restriction enzyme | Band size (bp) |
|---|---|---|---|---|---|---|
| Factor V Leiden | 1691 G/A, rs6025 |
F: TCAGGCAGGAACAACACCAT R: GGTTACTTCAAGGACAAAATACCTGTAAAGCT |
241 | 58°C | Hind III | G = 241 A = 209, 32 |
|
| ||||||
| Prothrombin | 20210G/A, 3′UTR rs1799963 |
F: ATTGATCAGTTTGGAGAGTAGGGG R: AATAGCACTGGGAGCATTGAAGCT |
142 | 60°C | Hind III | G = 142 A = 119, 23 |
|
| ||||||
| TFPI | −536C/T, intron7 | F: TCTATTTTAATTGGCTGTAT R: GCATGATAATAGTTTCCTGG |
170 | 65°C | BseN1 | C = 170 T = 143, 27 |
|
| ||||||
| PAI-1 | 4G/5G, −675 promoter rs1799889 |
F: CACAGAGAGAGTCTGGCCACGT R: CCAACAGAGGACTCTTGGTCT |
98 | 60°C | Bsl I | 4G = 98 5G = 77, 22 |
|
| ||||||
| PAI-1 | −844G/A, 3′UTR rs2227631 |
F: CAGGCTCCCACTGATTCTAC R: GAGGGCTCTCTTGTGTCAAC |
510 | 60°C | XhoI | G = 510 A = 364, 146 |
|
| ||||||
| MTHFR | 677C/T, rs1801133 |
F: TGAAGGAGAAGGTGTCTGCGGGA R: AGGACGGTGCGGTGAGA |
198 | 62°C | Hinf I | C = 198 T = 175, 23 |
|
| ||||||
| MTHFR | 1298A/C, rs1801131 |
F: CTTTGGGGAGCTGAAGGACTACTAC F: CAATTTGTGACCATTCCGGTTTG |
163 | 62°C | MboII | A = 56, 31, 30, 28, 18 C = 84, 31, 30, 18 |
|
| ||||||
| Fibrinogen-β | 148C/T, promoter rs1800787 |
F: CCTAACTTCCCATCATTTTGTCCAATAAA R: TGTCGTTGACACCTTGGGACTTAACTAG |
362 | 53°C | Hind III | C = 265, 97 T = 362 |
|
| ||||||
| Fibrinogen-β | −455G/A, promoter rs18000790 |
F: GCTTGTGGGAAATGAAGGAA R: GGCAACCACTAAAATCGTGA |
469 | 59.5°C | HaeIII | A = 469, 26 G = 383, 86, 26 |
PCR1-RFLP details showing primer sequences, PCR product size, annealing temperature, restriction enzyme, digestion temperature, and the fragment sizes.