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. 2014 Oct 8;11:52. doi: 10.1186/s12989-014-0052-6

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© Silva et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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At Day 1, Ag NW instillation produced marked inflammation in regions adjacent to particle agglomerates. Images are from serial tissue sections stained with H & E (left) or CEM (right) stains, and recovered at 1 day post exposure to sham control (A-B), S-Ag NWs (C-D), or L-Ag NWs (E-F) Panels are Brightfield microscopy images of tissues from rats instilled with a single 0.5 ml/kg dose of sham control or Ag NWs (indicated by solid arrows). Broken arrows indicate eosinophils, which are bright pink in H & E and CEM panels. BV = blood vessel, and TB-ADJ = terminal bronchiole-alveolar duct junction. Scale bar is 50 μm.