Table 1.
Genes in the predicted pectinase gene cluster in Caldicellulosiruptor bescii
| Gene | Predicted gene product | CAZy module architecture | Gene location/size |
|---|---|---|---|
| pecA (Cbes1855) | Pectate disaccharide lyase (EC:4.2.2.9) | SP-CBM_4_9-PL9 | 1933527-1935488/1.962 kb |
| pecB (Cbes1854) | Pectate lyase (EC:4.2.2.2) | SP-CBM_4_9-PL3 | 1932088-1933470/1.383 kb |
| pecC (Cbes1853) | Rhamnogalacturonan lyase | SP-PL11-CBM3 | 1929397-1931898/2.502 kb |
| pecR (Cbes1856) | AraC family transcriptional regulator | 1935834-1938215/2.382 kb |
Cbes1853, 1854, and 1855 are predicted to encode extracellular CAZy proteins [19] which contain signal peptides at the 5’- end and a CBM. The abbreviations are: CAZy, carbohydrate active enzyme; EC, enzyme commission number; SP, signal peptide; PL3, PL9, and PL11, polysaccharide lyase family of 3, 9, and 11; CBM, carbohydrate-binding module; CBM3, carbohydrate-binding module of family 3; CBM_4_9, putative pectin-binding module [12,13].