Table 1.
MALDI-TOF-MS results of picked spots from control, drought treatment and recovery of drought tolerant, # 11434, and susceptible, # 11431, genotypes and their expression values in comparison to control. The search carried out against the entire NCBInr database; SID: spot identification number; Mr/pI: calculated molecular weight and isoelectric point of predicted proteins; S: Score; *protein scores greater than 84 are significant (P < 0.05); C%: percentage of coverage; M: number of peptides matched; D: drought; R: recovery.
| Spot ID | NCBI Acc. no. | Protein name | Mr/pI | S* | %C | M | # 11434 | # 11431 | ||
|---|---|---|---|---|---|---|---|---|---|---|
| D | R | D | R | |||||||
| 01: Metabolism | ||||||||||
| 6 | gi/18483235 | Methionine synthase/Sorghum bicolor | 84135/5.93 | 96 | 8% | 8 | 6.7 | 1.6 | 6.6 | |
| 7 | gi/18483235 | Methionine synthase/Sorghum bicolor | 84135/5.93 | 102 | 12% | 12 | 5.5 | |||
| 16 | gi/226502947 | S-adenosylmethionine synthase l Z. mays | 42986/5.57 | 193 | 19% | 10 | 1.7 | 1.5 | ||
| 29 | gi/666089 | P-(S)-hydroxymandelonitrile lyase Sorghum bicolor | 41796/4.72 | 154 | 13% | 8 | 3.6 | |||
| 02: Energy | ||||||||||
| 1 | gi/242096062 | Hypothetical protein Sorbidraft of S. bicolor homologues to putative C4 phosphoenolpyruvate carboxylase/Saccharum officinarum CAC08829.1 |
108988/5.89 | 306 | 29% | 28 | 2.1 | 3.3 | −2 | |
| 2 | gi/242096062 | Hypothetical protein Sorbidraft of S. bicolor homologues to putative C4 phosphoenolpyruvate carboxylase/S. officinarum CAC08829.1 |
108988/5.89 | 504 | 30% | 33 | 1.7 | |||
| 3 | gi/242096062 | Hypothetical protein Sorbidraft of S. bicolor homologues to putative C4 phosphoenolpyruvate carboxylase/S. officinarum CAC08829.1 |
108988/5.89 | 331 | 29% | 30 | 2.3 | −2 | ||
| 4 | gi/242096062 | Hypothetical protein Sorbidraft of S. bicolor homologues to putative C4 phosphoenolpyruvate carboxylase/S. officinarum CAC08829.1 |
108988/5.89 | 354 | 26% | 30 | 2.4 | |||
| 5 | gi/242080811 | Hypothetical protein Sorbidraft of S. bicolor homologues to aconitate hydratase of Oryza sativa Japonica Q6YZX6.1 |
108402/6.41 | 101 | 11% | 13 | 1.8 | |||
| 9 | gi/242051769 | Hypothetical protein Sorbidraft of S. bicolor homologues to chloroplast NADP-dependent malic enzyme Z. mays NP_001105313.1 |
69904/6.23 | 181 | 28% | 21 | −2.8 | −2.4 | ||
| 10 | gi/242051769 | Hypothetical protein Sorbidraft of S. bicolor homologues to chloroplast NADP-dependent malic enzyme Z. mays NP_001105313.1 |
69904/6.23 | 199 | 25% | 18 | −2.8 | −2.7 | ||
| 11 | gi/242051769 | Hypothetical protein Sorbidraft of S. bicolor homologues to chloroplast NADP-dependent malic enzyme Z. mays NP_001105313.1 |
69904/6.23 | 193 | 26% | 18 | −2.8 | −2.3 | −2 | |
| 17 | gi/242059597 | Hypothetical protein Sorbidraft of S. bicolor homologues to fructose-1,6-bisphosphat aldolase, cytosol Z. mays NP_001105336.1 |
38990/6.96 | 278 | 23% | 12 | 1.6 | |||
| 23 | gi∣195634659 | Plastid fructose-1,6-bisphosphate aldolase/Z. mays | 41924/7.63 | 607 | 37% | 22 | −2.2 | −1.9 | −2.4 | |
| 24 | gi∣195634659 | Plastid fructose-1,6-bisphosphate aldolase, Z. mays | 41924/7.63 | 395 | 34% | 19 | −2.1 | −1.9 | −1.8 | |
| 19 | gi∣108705994 | Plastid glyceraldehyde-3-phosphate dehydrogenase/O. sativa Japonica | 34024/4.99 | 121 | 19% | 11 | −1.5 | |||
| 20 | gi/255540341 | Cytosolic glyceraldehyde-3-phosphate dehydrogenase/Ricinus communis | 36930/7.10 | 157 | 20% | 14 | 1.6 | 1.7 | 1.7 | |
| 30 | gi/30385668 | Pyruvate phosphate dikinase/Sorghum bicolor | 103021/5.68 | 94 | 16% | 17 | 1.9 | 1.6 | ||
| 04: Transcription | ||||||||||
| 21 | gi/242085078 | Hypothetical protein Sorbidraft of S. bicolor homologues to RNA-binding protein/Arabidopsis thaliana NP_172405.1 |
42324/8.89 | 438 | 40% | 25 | −2 | |||
| 28 | gi∣195637410 | 40S ribosomal protein S3/Z. mays | 25605/9.4 | 117 | 46% | 12 | 1.8 | |||
| 05: Protein synthesis | ||||||||||
| 15 | gi∣195620072 | Elongation factor alpha/Z. mays | 49534/9.15 | 225 | 21% | 14 | 8.5 | 5 | 7 | |
| 06: Protein destination/storage | ||||||||||
| 8 | gi∣162459902 | Nucleoredoxin 1/Z. mays | 64058/4.8 | 168 | 16% | 12 | 4.4 | 4.9 | 4.5 | |
| 12 | gi/242094438 | Hypothetical protein Sorbidraft of S. bicolor homologues to Heat shock protein 60 | 61927/5.47 | 148 | 31% | 16 | 2.1 | |||
| 13 | gi/242094438 | Hypothetical protein Sorbidraft of S. bicolor homologues to Heat shock protein 60 | 61927/5.47 | 161 | 29% | 14 | 2.2 | |||
| 14 | gi/242094438 | Hypothetical protein Sorbidraft of S. bicolor homologues to Heat shock protein 60 | 61927/5.47 | 126 | 19% | 12 | 1.9 | 2.3 | ||
| 18 | gi/242041951 | Hypothetical protein Sorbidraft of S. bicolor homologues to pepsin/retropepsin like aspartat protease/Z. mays ACG35399.1 |
42799/8.96 | 79 | 14% | 2 | ||||
| 31 | gi/242056107 | Hypothetical protein Sorbidraft of S. bicolor homologues to mitocondrial processing peptidase/Z. mays NP_001150614.1 |
54054/6.24 | 198 | 28% | 12 | 1.7 | |||
| 32 | gi/145666464 | Protein disulfide isomerase/Z. mays | 56921/5.01 | 174 | 19% | 18 | 2.1 | 2.5 | ||
| 12: Unclear classification | ||||||||||
| 22 | gi/242075782 | Hypothetical protein Sorbidraft of S. bicolor homologues to ABA stress- and fruit-ripening inducible-like protein Z. mays CAA72998.1 |
28466/4.92 | 258 | 65% | 22 | 3.6 | 4.5 | 7 | |
| 25 | gi/242035869 | Osr40Sc1 like protein | 39718/6.27 | 66 | 9% | 3 | 5.2 | |||
| 26 | gi/242035869 | Osr40Sc1 like protein | 39718/6.27 | 201 | 13% | 6 | 3.6 | 3.3 | 3 | |
| 27 | gi/242056773 | Hypothetical protein Sorbidraft/S. bicolor | 31577/6.06 | 205 | 23% | 9 | 3.6 | 2.9 | 4.6 | |
| 33 | gi/242095250 | Hypothetical protein Sorbidraft/S. bicolor | 41333/4.91 | 222 | 37% | 14 | 1.9 | 2.3 | ||