FIG. 3.

Correlation between heat-inducible binding of HSF and mRNA expression. (A) Verification of HSF binding to selected targets by PCR. The same IP DNA used for microarrays as shown in Fig. 2B was used to verify binding of HSF to selected promoters by PCR. SSA1, HSP82, and SSA3 were previously known targets of HSF, and the remainder were targets newly identified in this study. The PHO5 promoter was used as a negative control. WCE, whole-cell extract. (B) Kinetics of HSF target gene expression by heat shock. Expression levels for the indicated genes were detected by RNA blot analysis. ENO1 was used as a negative control.