FIG. 5.

Validation of microarray results. Promoter-specific PCR for the indicated genes was performed using ChIP samples that had earlier been analyzed on microarrays. (A) Genes predicted as targets by microarrays and containing a consensus HSE in their promoter. (B) Genes predicted as targets by microarrays and lacking a consensus HSE in their promoter. (C) Genes identified as nontargets by microarrays, containing a consensus HSE in their promoter. ILV2, ATC1, ICS2, and BSC5 were not appreciably induced by heat shock. WCE, whole-cell extract.