FIG. 6.

KAKTLR is defective for adhesion dependent tyrosine phosphorylation. CE cells expressing wild-type FAK (lanes 1 to 5) or KAKTLR (lanes 6 to 10) were held in suspension or plated onto fibronectin for the indicated times. Tyrosine phosphorylation in subconfluent cells in culture was also examined (lanes 1 and 6). FAK or KAKTLR was immunoprecipitated and analyzed by Western blotting for phosphotyrosine (pTyr) (panel A, top panel). Tyrosine phosphorylation at position 397 was examined by Western blotting of whole cell lysates with PY397 (panel B, top panel). As a loading control, these blots were stripped and reprobed with BC4 (bottom panels).