FIG. 8.

KAKTLR is defective for signaling in vivo. (A) Lysates of CE cells expressing empty RCAS A (lanes 1 and 2), FAK (lanes 3 and 4), or KAKTLR (lanes 5 and 6) were analyzed by Western blotting for phosphotyrosine (pTyr). Prior to lysis, cells were either left untreated or treated with 50 μM vanadate (VO₄) for 16 h. (B) Endogenous paxillin was immunoprecipitated from lysates of control cells (lanes 1 and 2) or cells expressing FAK (lanes 3 and 4) or KAKTLR (lanes 5 and 6) treated as for panel A. Tyrosine phosphorylation was examined by Western blotting (top). The blot was stripped and reprobed with a monoclonal antibody recognizing paxillin (bottom).