Table 1.

The main algorithms for computational target prediction.

Algorithm	Website	Last Update	Input	Organisms	Features	References
miRanda	www.microrna.org	August 2010	miRNA name, miRNA sequence, gene name	human, rat, mouse, fly, worm	seed match, conservation, free energy	Enright et al., 2003 [40] John et al., 2004 [23]
TargetScan	www.targetscan.org	June 2012	miRNA name, miRNA family, gene name	mammals, fly, worm	seed match, conservation	Lewis et al., 2005 [41] Grimson et al., 2007 [24] Friedman et al., 2009 [42] Garcia et al., 2011 [43]
DIANA-microT-CDS	www.microrna.gr/microT-CDS	July 2012	miRNA name, gene name, Ensembl ID	human, mouse, fly, worm	seed match, conservation, free energy, site accessibility, target-site abundance	Maragkakis et al., 2009 [44] Reczko et al., 2012 [45] Paraskevopoulou et al., 2013 [46]
PicTar	pictar.mdc-berlin.de	March 2007	microRNA name, gene name	vertebrate, fly, worm, mouse	seed match, pairing stability	Lall et al., 2006 [47]