FIG. 8.

Partial substitution of ORF5 for LAT function in TCR signal transduction. (Top) Intracellular calcium mobilization. J.CaM2.5 LAT-deficient cells electroporated with pTracerEF.GFP, pTracerEF.GFP-ORF5, or pTracerEF.GFP-LAT were stimulated with anti-CD3 (αCD3) Dynabeads, and calcium mobilization in GFP-positive cell populations was then monitored over time. The procedure and data presentation are as described for Fig. 7A. Data were similar in two independent experiments. (Bottom) Surface expression of CD69. At 24 h postelectroporation with pTracerEF.GFP or pTracerEF.GFP-ORF5, J. CaM2.5 cells were unstimulated or stimulated with either anti-CD3 Dynabeads or PMA (50 ng/ml) for an additional 16 h. GFP-positive cell populations were then gated and examined for CD69 surface expression by flow cytometry.