Fig. 5.

Effects of RCM extracts on NF-κB activity (A), IκB phosphorylation (B) and MAP kinase activity (C) in LPS induced RAW 264.7. Cells were treated with UE, UH, RE and RH (400 µg/ml) for 24 h then stimulated with LPS (1 µg/ml) for 20 min (A, C) or 15 min (B). NF-κB activity and relative IκB phospholylation were measured by NF-κB (p65) transcription factor assay kit (A) and western blot (B) and MAP kinase activity was measured by western blot (C). Values not sharing the same letter were significantly different (P < 0.05). (UE: unripe Rubus coreanus Miquel ethanol extract, UH: unripe Rubus coreanus Miquel water extract, RE: ripe Rubus coreanus Miquel ethanol extract, and RH: ripe Rubus coreanus Miquel water extract)