FIG. 5.

Telomeric G overhang analysis of mVparp^−/− mouse ES cells and tissues. (A) Genomic DNA from wild-type and mVparp^−/− ES cells and mouse liver was resolved by pulsed-field gel electrophoresis and subjected to in-gel hybridization to a radioactively labeled (TTAGGG)₄ probe under native conditions. In lane 1, a denatured genomic DNA sample from wild-type mouse liver (which was excised from the same gel and subsequently denatured) was used as a positive control for hybridization. Since the telomeric G overhang consists of TTAGGG repeats, no hybridization should be evident in lanes 2 to 9. MBN, samples that were pretreated with mung bean nuclease prior to electrophoresis. (B) The same samples as those in panel A were hybridized to a radioactively labeled (CCCTAA)₄ probe. (C) The same gel as that in panel B was denatured and reprobed with a radioactively labeled (CCCTAA)₄ probe. The integrity of the high-molecular-weight DNA was confirmed by ethidium bromide staining of the agarose gel prior to hybridization (data not shown). DNA markers (kilobase pairs) are indicated at the right.