FIG. 6.

Vault structure and copurification of vault components in mVparp^−/− mouse livers. (A) Electron micrographs show negatively stained vaults purified from livers of mVparp^−/−, mVparp^+/−, and wild-type (+/+) mice. Bar, 50 nm. (B) Purified vaults were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by silver staining. (C) The identities of the vault components (TEP1, VPARP, and MVP) were confirmed by immunoblot analysis with anti-TEP1, anti-VPARP, and anti-MVP antibodies. As expected, VPARP was absent from mVparp^−/− mice.