Figure 2.

Influence of active substances alone or in combination on estradiol production and CYP19 mRNA expression in Jeg-3 cells. Jeg-3 cells were grown in 6-well cell culture plates for 24 h. Thereafter, the cells were incubated with the substances in total concentrations of 30 and 40 µM as indicated for 48 h individually and in combination: (A) 30 µM for epoxiconazole, tebuconazole and flusilazole, (B) 40 µM for epoxiconazole, cyproconazole and prochloraz, (C) 40 µM for prochloraz, chlorpyrifos and triflusulfuron-methyl. The supernatant was collected and estradiol concentration measured by ELISA as indicated by the manufacturer’s protocol. The RNA of the cells was isolated and reverse transcription performed as described. The data represent the mean for three independent experiments. * Indicates significance with p ≤ 0.05.