Figure 3.

Scheme of expression of steroid biosynthesis relevant genes and supernatant hormone concentration of progesterone and estradiol in trophoblasts based on experiments in Jeg-3 cells. Jeg-3 cells were treated with either 30 µM or 40 µM (as indicated) of cyproconazole, chlorpyrifos, epoxiconazole, flusilazole, prochloraz, tebuconazole and triflusulfuron-methyl individually and in combination for 48 hours. Gene expression was analysed by quantitative real-time PCR. Progesterone and estradiol were measured using commercial ELISA systems. Key on the right side indicates the order of presentation for the concentration groups as well as significant increase/upregulation and decrease/downregulation. The data represent the mean ± SD for three independent experiments. Concentration values given for combination treatments reflect total concentrations of all three components.