Figure 5.

Substance effects on AhR transactivation. Jeg-3 cells were grown in 96-well cell culture plates for 24 h. Thereafter, the cells were transiently transfected with xenobiotic response element (XRE) luciferase reporter gene and renilla reporter gene (40:1) and TransIT-2020 in a ratio 1:3 for 20 h. Finally, the cells were incubated with the substances individually and in combination in concentrations as indicated: (A) flusilazole, epoxiconazole and tebuconazole, (B) epoxiconazole, cyproconazole and prochloraz, (C) prochloraz, chlorpyrifos, triflusulfuron-methyl. Cells were lysed and luciferase activity was measured as indicated by the manufacturer. The data represent the mean ± SD for two independent experiments (n = 6). Concentration values given for combination treatments reflect total concentrations for all three components. Concentrations are given in µM.