Figure 3.

An equal versus unequal distribution of the apical membrane is not necessarily associated with a vertical versus non-vertical cleavage plane of NE cells. Frozen sections of E9.5–10.0 mouse hindbrain neuroepithelium were double-labeled for cadherin (A–C; red) and DNA (A–C; blue; propidium iodide staining). In the single optical section shown, white bars indicate the apical membrane (cadherin hole), and the dashed white lines show the cleavage plane deduced as described in Figure 2A. Note that in (B), the cleavage plane is vertical but the apical membrane distributed unequally, whereas in (C), the cleavage plane is oblique but the apical membrane is distributed equally. The asterisk in (B) indicates the cadherin hole of another cell adjacent to the mitotic NE cell shown.