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Estrogen antagonists enhance the transcriptional activity of the prohibitin promoter. MCF7 and ZR75-1 cells were transfected with ProhibitinCAT, a CAT reporter gene driven by a rat prohibitin promoter element spanning −485 to −5 bp. Cells were treated with 4HT or ICI182780 for the indicated time intervals, and harvested for CAT activity assay and β-galactosidase assay (transfection control).
