Table 2.
Reversal of estrogen antagonist-induced repression of colony formation by Brg1 SiRNA and Brm SiRNA
| Vector-based SiRNA transfection | Number of colonies |
|||
|---|---|---|---|---|
| MCF7 |
ZR-75-1 |
|||
| Experiment 1 | Experiment 2 | Experiment 3 | Experiment 4 | |
| Control SiRNA+ethanol | 245 | 243 | 269 | 265 |
| Brg1 SiRNA+ethanol | 255 | 241 | 272 | 271 |
| Brm SiRNA+ethanol | 242 | 246 | 261 | 261 |
| Control SiRNA+4HT | 5 | 3 | 11 | 12 |
| Brg1 SiRNA+4HT | 263 | 258 | 275 | 261 |
| Brm SiRNA+4HT | 239 | 244 | 256 | 251 |
| Control SiRNA+ICI | 7 | 5 | 8 | 6 |
| Brg1 SiRNA+ICI | 238 | 244 | 254 | 255 |
| Brm SiRNA+ICI |
249 |
240 |
261 |
258 |
| Approximately 10 000 MCF7 or ZR-75-1 cells were transfected with 10 μg of the indicated vector-based SiRNA (in pRNAT-U6.1/Neo). Colonies with 20 or more cells were scored after 14 days of selection in 100 μg of neomycin per ml. | ||||