Figure 2.

MOF associates with the DCC through a stable interaction with MSL1. SF9 cells were coinfected with various baculoviruses expressing HA-tagged MOF (HA-MOF), MSL1, MSL2 and flag-tagged MSL3 in the combinations indicated by ‘+' above each lane. Protein complexes were purified from total cell extracts by chromatography on beads coated with flag- or HA-specific antibodies (flag beads, HA beads, respectively). After binding, the beads were washed stringently (1 M NaCl, 1% NP40), and the adsorbed proteins were analysed by SDS–PAGE and Coomassie blue staining. The lane marked ‘M' displays protein size standards in kDa (206, 119, 91 and 51.4 kDa, respectively).