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MSL1 and MSL3 together promote the binding of MOF to DNA and chromatin. Free (D) or nucleosomal (N) linear DNA was immobilized on paramagnetic beads and incubated either with MOF alone (lanes 5 and 6) or MOF and flag-MSL3 (lanes 7 and 8), MOF and GST-MSL1 (lanes 9 and 10), or all three proteins (lanes 11 and 12). After washes, the bound proteins were detected by Western blots using specific antibodies. Input: 5% of input (lanes 1–4); bound: 50% of bound proteins (lanes 5–12).
